Nita Ynniar ningsih / 1600017101
Restriction Enzymes: DNA Scissors
Restriction Enzymes
Directions: Identify the restriction sites for each
of the examples given. Show the cuts , sticky ends or blunt, number
of DNA fragments produced and the number of base pairs in each (count
the top row). (If there are three nucleotides on either side of the dash it is a
blunt cut. If there are fewer than three bases on either side of the dash it produces
“sticky ends” cut.)
Remember: Analyze in the 5’à 3’ direction
1. HindIII--- 5' GTC ↓GAC 3'
5' ACGACGTAGTCGACTTATTAT
GTCGACCCGCCGCGTGTCGACCATCA 3'
3' TGCTGCATCAGCTGAATAATACAGCTGGGCGGCGCACAGCTGGTAGT
5'
Number of pieces of
DNA ___3____
Tipe pemotongan : ujung timpul (blunt ends)
Jumlah pemotongan fragmen DNA : 4 fragmen
Titik potong : 3 bagian
Jumlah pasangan basa nitrogen : 15 bp, 13 bp, 11 bp, dan 8 bp.
2. EcoRI --- 5' G ↓AATTC 3'
5' ACG ACGTATTAGAATTCTTAT
CCGCCGCCGGAATTCT CATCA 3'
3' TGC TGCATAATCTTAAGAATAGGCGGCGGCCTTAAGAGTAGT
5'
Number of pieces of
DNA ____2___
Tipe pemotongan : ujung lancip (sticky ends)
Jumlah hasil pemotongan fragmen DNA : 3 fragmen
Titik potong : 2 bagian
Jumlah pasangan basa nitrogen : 19 bp, 12 bp, dan 11 bp.
3. HaeIII --- 5' CC ↓ GG 3'
5' ACGCCGGCCGTATTAT
CCGGATCCGCCG CCGGCTGTCCCGGATCA 3'
3' TGCGGCCGGCATAATAGGCCTAGGCGGCGGCCGACAGGGCCTAGT
5'
Number of pieces of
DNA ___4____
Tipe pemotongan : ujung tumpul (blunt ends)
Jumlah hasil pemotongan fragmen DNA : 5 fragmen
Titik potong : 4 bagian
Jumlah pasangan basa nitrogen : 13 bp, 12 bp, 9 bp, 6 bp, dan 5 bp.
4. BamI --- 5' CCTAG ↓G 3'
5' ACGCCTAGGACGTATTATCCTAGGTAT
CCGCCGCCGT CATCA 3'
3' TGCGGATCCTGCATAATAGGATCCATAGGCGGCGGCAGTAGT
5'
Number of pieces of
DNA ___2____
Tipe pemotongan : ujung lancip (sticky ends)
Jumlah hasil pemotongan fragmen DNA : 3 fragmen
Titik potong : 2 bagian
Jumlah pasangan basa nitrogen : 19 bp, 15 bp, dan 8 bp.
5. HindII --- 5' GTC ↓- GAC 3' and HaeIII
--- 5' CC ↓ GG 3'
5' ACGGTCGACACGTATTATTAGTCGACTCCGCCGCCGCCGGTCATCA
3'
3' TGCCAGCTGTGCATAATAATCAGCTGAGGCGGCGGCGGCCAGTAGT
5'
Number of pieces of
DNA ___3___
Tipe pemotongan : ujung tumpul
(blunt ends)
Jumlah hasil pemotongan fragmen DNA : 4 fragmen
Titik potong : 3 bagian
Jumlah pasangan basa nitrogen : 17 bp, 15 bp, 8 bp dan 6 bp.
6. HindII --- 5' GTC ↓ GAC 3', HaeIII
--- 5' CC ↓ GG 3' and BamI --- 5'
CCTAG ↓ G 3'
5' ACGCCGGACGTACCTAGGTTTAGTCGACTC
CGCCG CCCCTAGGGTCATCA 3'
3' TGCGGCCTGCATGGATCCAAATCAGCTGAGGCGGCGGGGATCCCAGTAGT
5'
Number of pieces of
DNA ____4___
Tipe pemotongan : ujung
tumpul (blunt ends)
Jumlah hasil
pemotongan fragmen DNA : 4 fragmen
Titik potong : 3
bagian
Jumlah pasangan
basa nitrogen : 20 bp, 17 bp, 8 bp dan 5 bp.